id inhibitor dmh2  (MedChemExpress)

Journal: Cell reports

Article Title: Fate erasure logic of gene networks underlying direct neuronal conversion of somatic cells by microRNAs

doi: 10.1016/j.celrep.2024.115153

Figure Lengend Snippet:

Article Snippet: miRCURY LNA miRNA Power Inhibitors (HSA-LET-7C-5P: GeneGlobe Cat# YI04100671-DCA; NS: GeneGlobe ID - YI00199006) , QIAGEN , Cat# 339130.

Techniques: Recombinant, Electron Microscopy, Staining, Illumina Sequencing, RNA Library Preparation, Imaging, Software

Journal: bioRxiv

Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen-tracks

doi: 10.1101/2025.01.25.634857

Figure Lengend Snippet: (a) Volcano map highlighting the 32 most expressed mRNAs enriched in collagen-tracks after RNA sequencing analysis (b) Venn diagram analysis of the 4 common proteins and mRNAs contained in collagen-tracks. (c) Bubble plot of the mRNAs related pathways. (d) Histogram of RNA binding RNA significantly present in collagen-tracks. Results are expressed in Log (ratio tracks/control). (e) Barplot of miRNAs enriched in collagen-tracks compared to control. (f) Histogram of the 7miRNAs significantly present in collagen-tracks and/or enriched into 1 to 3 replicates. Values represent the mean +/- SEM of n=3 independent experiments. (g) Bubble plot of the 7 miRNAs enriched and/or significantly present in collagen-tracks related pathways.

Article Snippet: miRCURY LNA miRNA Power Inhibitors (1 nmol) (hsa-let-7e-5p ID-YI04101354-DCA, hsa-miR-23a-5p ID-YI04101840-DCA, hsa-miR-98-5p YI04100423-DCA, hsa-miR-486-5p ID-YI04100385-DCA, hsa-miR-146a-5p ID-YI04100676-DCA, hsa-miR-450a-5p ID-YI04101851-DCA, hsa-let-7f-5p ID-YI04100302-DCA) and control (ID-YI00199006) were purchased from Qiagen.

Techniques: RNA Sequencing, RNA Binding Assay, Control

Journal: bioRxiv

Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen-tracks

doi: 10.1101/2025.01.25.634857

Figure Lengend Snippet: (a) Schematic procedure to form collagen-tracks without miRNAs of interest using miRNA inhibitors treatment. (b) mRNA expression levels of EMT genes in MCF-7 cells after internalization of collagen-tracks previously formed with anti-miRNAs treatment. The graph shows the quantification of VIM, MMP14 and ZEB1 mRNA expression levels normalized to 18S (Fold change). Values are expressed as the mean ± SEM of n=3 independent experiments. One-way ANOVA test followed by bonferroni test p<0.0001. (c) Representative immunofluorescence images of ECM degradation properties of MCF-7 cells after being treated with or without anti-miRNAs. Scale bar, 25 μm. (d) Quantification of ECM degradation properties of MCF-7 cells after being treated with or without anti-miRNAs. Values represent the mean +/- SEM of n=3 independent experiments (5 images per condition and per replicate) and were analyzed using One-way ANOVA test followed by bonferroni test p<0.0001.

Article Snippet: miRCURY LNA miRNA Power Inhibitors (1 nmol) (hsa-let-7e-5p ID-YI04101354-DCA, hsa-miR-23a-5p ID-YI04101840-DCA, hsa-miR-98-5p YI04100423-DCA, hsa-miR-486-5p ID-YI04100385-DCA, hsa-miR-146a-5p ID-YI04100676-DCA, hsa-miR-450a-5p ID-YI04101851-DCA, hsa-let-7f-5p ID-YI04100302-DCA) and control (ID-YI00199006) were purchased from Qiagen.

Techniques: Expressing, Immunofluorescence

Journal: bioRxiv

Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen-tracks

doi: 10.1101/2025.01.25.634857

Figure Lengend Snippet: (a) Immunofluorescence images of collagen-tracks after anti-miRNAs or anti-miRNA control treatment in MDA-DDR1-GFP cells. Cells were imaged by confocal microscopy. DDR1 is stained in green, actin in red, nuclei in blue and collagen is imaged by IRM in grey. Scale bar, 10 μm . (b) mRNA expression levels of EMT genes in MCF-7 cells after internalization of collagen-tracks previously formed with anti-miRNAs or anti-miRNA control. The graph shows the quantification of CDH1 , DDR1, S NAIL and ITGB1 mRNA expression levels normalized to 18S (Fold change). Values are expressed as the mean ± SEM of n=3 independent experiments and were analyzed using One-way ANOVA test p<0.001.

Article Snippet: miRCURY LNA miRNA Power Inhibitors (1 nmol) (hsa-let-7e-5p ID-YI04101354-DCA, hsa-miR-23a-5p ID-YI04101840-DCA, hsa-miR-98-5p YI04100423-DCA, hsa-miR-486-5p ID-YI04100385-DCA, hsa-miR-146a-5p ID-YI04100676-DCA, hsa-miR-450a-5p ID-YI04101851-DCA, hsa-let-7f-5p ID-YI04100302-DCA) and control (ID-YI00199006) were purchased from Qiagen.

Techniques: Immunofluorescence, Control, Confocal Microscopy, Staining, Expressing